Related Information:

This reagent card combines glass beadseparation technology, centrifugation, and the principles of serological redcell antigen-antibody agglutination. When anti-human globulin antibodies reactwith IgG adsorbed on red blood cells, agglutination occurs. Under centrifugalforce, the agglutinated red blood cells cannot pass through the gaps betweenthe glass beads and remain in the upper layer or are dispersed within theseparation medium of the microcolumn, indicating a positive reaction.Conversely, non-agglutinated red blood cells pass through the gaps and settleat the bottom of the microcolumn, indicating a negative reaction.

Agglutination Grading Standards:

• 4+: Red blood cells agglutinate on     the surface of the glass bead separation medium, forming a distinct ring.

• 3+: Most agglutinated red blood     cells are retained in the upper half of the glass bead separation medium.

• 2+: Agglutinated red blood cells     are distributed throughout the glass bead separation medium, with a small     amount settling at the bottom of the microcolumn.

• 1+: Most agglutinated red blood     cells are located in the lower half of the glass bead separation medium,     with more red blood cells at the bottom of the microcolumn.

• 0.5+: Most red blood cells pass     through the glass bead separation medium and form a rough (not smooth)     aggregation band at the bottom of the column, with a small number of red     blood cells above the aggregation band.

• Negative (-): Non-agglutinated red     blood cells pass through the gaps between the glass beads and form a     smooth aggregation band at the bottom of the microcolumn. In some cases, a     misty red coloration may appear above the smooth band due to     unagglutinated red blood cells, which is still considered negative.